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Alkaline Phosphatase Isoenzymes

Alkaline Phosphatase Isoenzymes

Alternate Test Name

Alkaline Phosphatase, Total and Isoenzymes, Serum

Epic Mnemonic
Sunquest Mnemonic

LAB741
ALKIB

Category

Sendouts

Methodology

Separation by Agarose Gel Electrophoresis

Test Performance Schedule

3 times a week

Result Availability

2 – 7 days

Specimen Required

Container

Gold (SST) or Red top (serum) tube

Volume

Pref. Vol.: 5.0 mL
Min. Vol.: 2.0 mL

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Clot 30 minutes
• Promptly centrifuge 15 minutes
• Immediately transfer to a plastic transport tube
• Refrigerate

Transportation Instructions

Refrigerated

Stability

After separation from cells:
• Room temperature: 2 hours
• Refrigerated: 7 days
• Frozen: n/a

Causes for Rejection

• Plasma specimens
• Grossly hemolyzed, lipemic or icteric specimens
• Red-top tubes with serum not separated from cells within two hours of collection
• Specimens with total Alkaline Phosphatase levels lower than111 U/L

Remarks

Patient’s age and sex are required

CPT Codes

84080, 84075

Effective/Revised

5/1/2023

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