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Vitamin D-1,25 Dihydroxy

Vitamin D-1,25 Dihydroxy

Alternate Test Name


1,25-Dihyroxyvitamin D, Serum; Calcitriol

Epic Mnemonic
Sunquest Mnemonic

LAB536
VIT2D

Category

Sendouts

Methodology

Chemiluminescence Immunoassay (CLIA)

Test Performance Schedule

Twice a week

Result Availability

2 - 7 days

Specimen Required

Container

Gold top (SST) tube

Volume

Pref. Vol.: 1.5 mL serum
Min. Vol.: 0.7 mL serum

Collection Instructions

• Routine venipuncture
Immediately after collection, gently invert tube 5-10 times
• Clot 30 minutes
• Promptly centrifuge 15 minutes
• Refrigerate

Transportation Instructions

Refrigerated

Stability

• Room temperature: 3 days
• Refrigerated (preferred): 7 days
• Frozen: 6 months

Causes for Rejection

Grossly hemolyzed specimen

Remarks

Calcitriol (1,25-Dihydroxyvitamin D) testing is not intended for routine monitoring of vitamin D levels. This test should be reserved for patients with renal disease, sarcoid tuberculosis, lymphoma and rickets, as well as, long term use of protease inhibitors, glucocorticoids, or anticonvulsants.For routine monitoring ofvitamin D levels in the majority of the population; Vitamin D 25 (25 OH D) is recommended.

CPT Codes

82652

Effective/Revised

5/1/2023

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