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Culture, Fungus

Culture, Fungus
Epic Mnemonic Sunquest Mnemonic	LAB240 FUNG
Category	Microbiology
Test Performance Schedule	Sunday - Saturday
Result Availability	Weekly negatives finalized at 28 days

Specimen Required

Container	Abscess, Body Fluids, CSF, Hair, Nails, Skin, Urine, Tissue: Sterile plastic container Blood: Aerobic BacT/Alert bottle Eye: Transport Media Sputum: Falcon collection system Bone Marrow: 1.5mL Isolator Genital & Wounds: Culture collection/transport system (eSwab)
Volume	Body Fluid: 5mL fluid CSF Min.: 3 mL
Collection Instructions	CSF: • Collected by lumbar, ventricular or cisternal puncture into sterile plastic tubes • Tube numbers should correspond to sample collection order • Culture will be performed on tube least likely to be contaminated with skin flora • Next to last tube collected preferred Eye: • Scrapings collected by physician and submitted on mini-tip culture swabs in transport media • Sterile swabs from culture collection/transport system Genital: • Sterile swabs from culture collection/transport system Hair: • Clip or pluck hair • Place in sterile container • A Wood's lamp is useful in the collection of specimens in tinea capitis infections, since hairs infected by most members of the genus Microsporum frequently exhibit fluorescence under a Wood's lamp. However, in tinea capitis due to Trichophyton sp., infected hairs usually do not fluoresce Nails, Skin: • Wash site with 70% alcohol • Allow to dry Tissue: • Keep moist by wrapping in gauze and adding a small amount of sterile water or saline Urine: • Early morning specimen Suspected Ringworm Cases: • Skin scrapings should be collected at outer periphery or border of lesion Sputum: • Three first morning specimens submitted on three successive days recommended Culture Collection Guide
Transportation Instructions	• Room temperature
Stability	72 hours, <24 hours recommended
Causes for Rejection	• Not received in appropriate collection and transport container • Dry swab • Improperly collected specimen • Delay in transport to the laboratory >72 hours
CPT Codes	87102
Effective/Revised	2/10/21

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Culture, Fungus