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Bilirubin, Direct

Bilirubin, Direct

Alternate Test Name

Conjugated Bilirubin

Epic Mnemonic
Sunquest Mnemonic

LAB52
DBIL

Category

Chemistry

Methodology

Colorimetric; Diazotization

Test Performance Schedule

Sunday - Saturday

Result Availability

Within 24 hours – STAT upon request

Specimen Required

Container

Gold top (SST), Red top (serum), or Green top (lithium heparin) tube

Volume

Pref. Vol.: 1.0 mL serum or plasma
Min. Vol.: 0.5 mL serum or plasma

Collection Instructions

• Routine venipuncture

• Immediately after collection, gently invert tube 5-10 times

• Avoid exposure to light

• Clot 30 minutes

• Promptly centrifuge 10 minutes

• If no gel barrier is present immediately transfer serum or plasma to a plastic tube and refrigerate

• Properly centrifuged gel barrier tube does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Room Temperature: 2 days
• Refrigerated: 5 days

Causes for Rejection

• Hemolysis

• Prolonged exposure to light

CPT Codes

82248

Effective/Revised

03/22/2017

Clinical Significance

Bilirubin is an organic compound formed by the reticuloendothelial system during the normal and abnormal destruction of red blood cells. Measurements of bilirubin are used in the diagnosis of liver disease, in the detection of hemolytic anemia, and to evaluate degrees of jaundice.

 

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