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HIV-1 RNA, Quantitative PCR

HIV-1 RNA, Quantitative PCR
Alternate Test Name	Human Immunodeficiency Virus; HIV-1 RNA; HIV-1 RNA, RT-PCR Quantitative Ultrasensitive
Epic Mnemonic Sunquest Mnemonic	LAB918 HVPCRB
Category	Tuesday, Friday
Methodology	Real-Time Polymerase Chain Reaction (RT-PCR)
Test Performance Schedule	Available once per week
Result Availability	1 – 7 days

Specimen Required

Container	Lavender top (EDTA) tube
Volume	Pref. Vol.: 6.0 mL whole blood collected in EDTA tube(s) Min. Vol.: 1.6 mL EDTA plasma
Collection Instructions	• Routine venipuncture • Immediately after collection, gently invert tube 5-10 times • Promptly centrifuge 20 minutes • Promptly transfer plasma to plastic transport tube • Freeze
Transportation Instructions	Frozen
Stability	After separation from cells: • Room temperature: 24 hours • Refrigerated: 5 days • Frozen (preferred): 60 days
Causes for Rejection	• Specimen not centrifuged within 6 hours of collection time • Specimen collected in tube containing gel barrier material • Plasma not transferred to sterile polypropylene tube • Specimen not transported frozen
Remarks
CPT Codes	87536
Effective/Revised	8/27/2019

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