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Antinuclear IgG Antibody Titer and Pattern, IFA

Antinuclear IgG Antibody Pattern and Titer, IFA

Alternate Test Name

ANA (Antinuclear Antibodies), Hep2 Substrate IFA with reflex to Titer and Pattern if positive

Epic Mnemonic
Sunquest Mnemonic

LAB2004
ANAIFA

Category

Sendouts

Methodology

ANA Hep2 slide IFA

Test Performance Schedule

Performed 3x per week

Result Availability

1 - 4 days

Specimen Required

Container

Gold top (SST) tube

Volume

Pref. Vol.: 5.0 mL serum
Min. Vol.: 2.0 mL serum

Collection Instructions

• Routine venipuncture
• Immediately after collection, gently invert tube 5-10 times
• Clot 30 minutes
• Promptly centrifuge 15 minutes
• Properly centrifuged gel barrier sample does not require transfer of serum or plasma to separate tube

Transportation Instructions

Refrigerated

Stability

• Room temperature: 8 hours
• Refrigerated: 7 days
• Frozen: 1 month

Causes for Rejection

Specimen not centrifuged within 2 hours

Remarks

Preferred confirmatory ANA test for autoimmune connective tissue diseases

CPT Codes

86038

Effective/Revised

8/16/2019

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